Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method

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Antibacterial Activity Assessment of Textile Materials: Check pH and adjust to 7. Other recommended strains are listed below in Section 6. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment.

This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use. Users of this test method are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility.

If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed A 50 TM Copyright? Report of results will include an observation of zones of inhibition and growth under the specimen if present.


Allow agar to gel firmly before inoculating.

BoxManassas VA ; tel: The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria. Take care not to metgod the surface of the agar while making the streaks. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.

The test is followed in the cases where the antibacterial agent is migratory i.

Uses and Limitations 1. This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn.

Antimicrobial – Parallel Streak Method AATCC 147

AATCC grants you a license as follows: Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center. Precision and Bias 7.

AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium. These safety precautions are for ttest purposes only. The hard copy print may only be distributed to other employees for their internal use within your organization.

Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide.

This test method is not for resale. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise.

To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen.


May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this. At the conclusion of two weeks, make a fresh transplant from stock culture. Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.

AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which aatcd adequately sensitive but is cumbersome and etst consuming for routine quality control and screening tests. Adjust to pH 6. Until a precision statement trst generated metthod this test method, use standard statistical techniques in making any comparisons of test results for either within-laboratory or between-laboratory averages.

All OSHA standards and rules must also be consulted and followed. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.

AATCC Antibacterial Parallel Streak Test Method

Maintenance of Culture of Test Organisms 8. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Notes and References